Kinetic Studies on Delignification and Heavy Metals Uptake by Shiitake (Lentinula edodes) Mushroom Cultivated on Agro-Industrial Wastes

This study investigates the sustainable production of Shiitake (Lentinula edodes) mushroom using agro-industrial wastes. The substrate (80% rice straw + 20% sugar cane bagasse) was moistened with 0 (freshwater as control), 50, and 100% concentrations secondarily treated dairy plant mill wastewaters (DPW SMW). After proper sterilization, cultivation carried out under controlled environmental conditions bag log method for 100 days. results revealed that DPW SMW moistening significantly (p < 0.05) increased nutrient levels formulated which later gave better yield. highest mycelial coverage (90.70 ± 1.47 88.65 1.82%), yield (186.00 3.10 176.09 4.12 g/kg fresh substrate), biological efficiency (80.00 0.58 75.73 0.93%), total phenol (2.84 0.03 2.69 mg/g), ascorbic acid (0.34 0.32 0.02 ?-carotene (2.48 0.06 2.29 ?g/g) contents minimum time taken spawn running (60 1 days) observed a 50% concentration treatment both SMW, respectively. Besides this, kinetic studies first-order-based model showed acceptable accuracy in predicting rate constant delignification heavy metal uptake by mushroom. These findings suggest novel approach concept can be used high-quality mushrooms edible medicinal purposes while contributing toward United Nations’ Sustainable Development Goals (SDGs 12) on responsible consumption superfoods.